Retour au sommaireMIZUKI I., TANI N., ISHIDA K., TSUMURA Y. 2005. Development and characterization of microsatellite markers in a clonal plant, Dioscorea japonica Thunb. Molecular Ecology Notes, 5: 721-723.
Abstract :
We developed 10 microsatellite loci from genomic DNA of a dioecious clonal plant, Dioscorea japonica. Out of 384 clones, 148 contained microsatellite repeats. Polymerase chain reaction primer pairs were designed for 95 of these clones from their sequence data, of which, 10 pairs produced successful amplification. Thirty-eight individuals were genotyped for allelic diversity. We detected three to nine alleles per locus, and the expected heterozygosity ranged from 0.461 to 0.851.